Chromatography of Conjugated Steroids on Lipophilic Sephadex.
نویسندگان
چکیده
منابع مشابه
Liquid-gel chromatography on lipophilic-hydrophobic Sephadex derivatives.
Hydrophobic long-chain alkyl ethers of Sephadex have been synthesized and tested for use in liquid chromatography. With columns prepared in such polar solvents as methanol, reversed-phase systems are obtained where compounds separate in order of decreasing polarity. In such non-polar solvents as heptane, straight-phase systems are formed, and separations occur in order of increasing polarity. T...
متن کاملPurification of human plasminogen and plasmin by gel filtration on Sephadex and chromatography on diethylaminoethyl-Sephadex.
Highly purified human plasminogen has been prepared from a variety of plasma fractions and by different purification methods. The two major fractions used have been serum or plasma euglobulin and Cohn plasma Fraction III. The purification methods (1-14) have all yielded products of high purity. Plasminogen useful for further purification and isolation studies can be prepared by the Kline method...
متن کاملSeparation of Folk Acid Compounds by Gel Chromatography on Sephadex
The separation of folate compounds has been achieved by gel chromatography and the effect of buffer concentration on the elution pattern of folate derivatives was investigated by Sephadex G-15 and G-25 column chromatography. Highly conjugated polyglutamates appear in the effluent considerably sooner when eluted with water than would be expected from their molecular weights. Monoglutamates, exce...
متن کاملPaper Chromatography of Steroids
In Paper I of this series (1) a method was described for the paper chromatographic analysis of certain ketonic steroids as hydrazones formed with Girard’s Reagent T. When that technique was applied to the adrenal cortical hormones, however, certain deficiencies appeared. (a) The RI values of the individual steroid-Reagent T hydrazones were not sufficiently different to permit identification. (b...
متن کاملThe Chromatography of Steroids
significant information in a given field and interpret it in terms of our current knowledge of the rapidly expanding research findings within the life sciences." The preceding publisher's statement is quoted because Dr. Sutton has so admirably carried out his mission. Genes are presented as functional units; specific examples of their fine structure are described with emphasis on their relation...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1966
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.20-1419